EFO:0003045

released
1{
2    "@context": "/terms/",
3    "@id": "/sample-terms/EFO_0003045/",
4    "@type": [
5        "SampleTerm",
6        "OntologyTerm",
7        "Item"
8    ],
9    "aliases": [
10        "igvf:H9"
11    ],
12    "ancestors": [
13        "early embryonic cell (metazoa)",
14        "cell",
15        "occurrent",
16        "precursor cell",
17        "process",
18        "experimentally modified cell in vitro",
19        "zygote",
20        "ESC derived cell line",
21        "conceptus",
22        "organism part",
23        "embryonic cell (metazoa)",
24        "multicellular organism",
25        "material entity",
26        "anatomical structure",
27        "cell type",
28        "animal zygote",
29        "processed material",
30        "multicellular anatomical structure",
31        "developmental stage",
32        "cultured cell",
33        "experimental factor",
34        "Homo sapiens cell line",
35        "embryo",
36        "entity",
37        "embryonic cell line",
38        "cell in vitro",
39        "material anatomical entity",
40        "Abstract human developmental anatomy",
41        "independent continuant",
42        "embryonic stem cell",
43        "cellular_component",
44        "continuant",
45        "anatomical entity",
46        "stem cell",
47        "anatomy basic component",
48        "single-cell tissue",
49        "stem cell derived cell line",
50        "eukaryotic cell",
51        "H9"
52    ],
53    "audit": {},
54    "cell_slims": [
55        "stem cell",
56        "embryonic cell"
57    ],
58    "comments": [],
59    "creation_timestamp": "2023-03-02T21:27:51.170552+00:00",
60    "definition": "This human ES cell line expresses cell surface markers specific to undifferentiated nonhuman primate ES and human EC cells: stage-specific embryonic antigen (SSEA)–3, SSEA-4, TRA-l-60, TRA-1- 81, and alkaline phosphatase. It has a normal 46, XX karyotype. Teratomas are formed upon cell injection into SCID mice. These teratomas include cells from all three germ layers.\n\nIsolation & Growth Conditions\nThis cell line can be cultured using feeder cells or in a feeder-free protocol. When using feeder cells, human ESC culture medium should be compreised of Advanced DMEM/F12 supplemented with knockout serum replacement (20%), non-essential amino acids (1x), L-glutamine (1x), penicillin/streptomycin (1x), β-mercaptoethanol (1x) and FGF-2 (4 ng/ml). Maintain the human ESCs on Matrigel using hESC medium conditioned by mouse embryonic fibroblasts.",
61    "developmental_slims": [],
62    "name": "EFO_0003045",
63    "ontology": "EFO",
64    "organ_slims": [
65        "embryo"
66    ],
67    "release_timestamp": "2023-03-02T23:03:52.524208+00:00",
68    "schema_version": "6",
69    "status": "released",
70    "submitted_by": {
71        "@id": "/users/43f2f757-5cbf-490a-9787-a1ee85a4cdcd/",
72        "title": "Jennifer Jou"
73    },
74    "summary": "H9",
75    "synonyms": [],
76    "system_slims": [],
77    "term_id": "EFO:0003045",
78    "term_name": "H9",
79    "uuid": "d6416afd-3d46-4752-aad4-6add0558063f"
80}