OBI:0001679

released
1{
2    "@context": "/terms/",
3    "@id": "/assay-terms/OBI_0001679/",
4    "@type": [
5        "AssayTerm",
6        "OntologyTerm",
7        "Item"
8    ],
9    "ancestors": [
10        "continuant",
11        "objective specification",
12        "assay",
13        "entity",
14        "information content entity",
15        "experimental factor",
16        "occurrent",
17        "directive information entity",
18        "experimental process",
19        "binding assay",
20        "planned process",
21        "yeast 2-hybrid assay",
22        "generically dependent continuant",
23        "protein and DNA interaction identification objective",
24        "process",
25        "sequence feature identification objective",
26        "biological feature identification objective"
27    ],
28    "assay_slims": [
29        "no category"
30    ],
31    "audit": {},
32    "category_slims": [
33        "protein and DNA interaction"
34    ],
35    "comments": [],
36    "creation_timestamp": "2023-09-26T20:08:04.729290+00:00",
37    "definition": "An assay that detects protein protein interactions and protein DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively. The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.",
38    "name": "OBI_0001679",
39    "objective_slims": [
40        "protein and DNA interaction identification objective"
41    ],
42    "ontology": "OBI",
43    "release_timestamp": "2023-11-22T19:12:37.476275+00:00",
44    "schema_version": "14",
45    "status": "released",
46    "submitted_by": {
47        "@id": "/users/6667a92a-d202-493a-8c7d-7a56d1380356/",
48        "title": "Khine Lin"
49    },
50    "summary": "yeast 2-hybrid assay",
51    "synonyms": [
52        "Y2H"
53    ],
54    "term_id": "OBI:0001679",
55    "term_name": "yeast 2-hybrid assay",
56    "uuid": "ba483776-fe27-4987-9e0f-1d10166122ee"
57}